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Optically Amplified RNA–Protein Detection Methods Using Light‐Harvesting Conjugated Polymers
Author(s) -
Wang S.,
Bazan G.C.
Publication year - 2003
Publication title -
advanced materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 10.707
H-Index - 527
eISSN - 1521-4095
pISSN - 0935-9648
DOI - 10.1002/adma.200305099
Subject(s) - conjugated system , förster resonance energy transfer , polymer , materials science , rna , chromophore , transactivation , fluorescence , nanotechnology , biophysics , photochemistry , chemistry , biology , biochemistry , gene expression , optics , physics , gene , composite material
A sensor is described that takes advantage of highly specific RNA–protein interactions and the light‐harvesting properties of conjugated polymers. Coordination between a cationic conjugated polymer (CP + ) and a negatively charged protein–RNA complex results in efficient Förster resonance energy transfer (FRET) from CP + to a protein‐bound chromophore (see Figure and inside cover). This sensor was used to detect the transactivation responsive RNA sequence of HIV‐1.