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Real‐Time PCR Test: All‐in‐One DNA Extraction Tube for Facilitated Real‐Time Detection of Infectious Pathogens (Adv. Healthcare Mater. 14/2021)
Author(s) -
Song Younseong,
Kim Yong Tae,
Choi Yunho,
Kim Hogi,
Yeom Min Hee,
Kim Yesol,
Lee Tae Jae,
Lee Kyoung G.,
Im Sung Gap
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202170067
Subject(s) - tube (container) , real time polymerase chain reaction , coating , polymerase chain reaction , pcr test , dna , materials science , dna extraction , virology , nanotechnology , chromatography , chemistry , medicine , biochemistry , gene , composite material
In article number 2100430 by Sung Gap Im, Kyoung G. Lee, and co‐workers, an “all‐in‐one tube” is devised by coating poly‐(2‐dimethylaminomethyl styrene) (pDMAMS) on a polymerase chain reaction (PCR) tube via initiated chemical vapor deposition (iCVD) to extract, amplify, and detect pathogens within a single tube. The all‐in‐one tube can capture the DNA of the pathogens by charge interaction and directly perform real‐time PCR, reducing the labor‐intensive pathogen detection steps.