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A 3D Bioprinted In Vitro Model of Pulmonary Artery Atresia to Evaluate Endothelial Cell Response to Microenvironment
Author(s) -
Tomov Martin L.,
Perez Lilanni,
Ning Liqun,
Chen Huang,
Jing Bowen,
Mingee Andrew,
Ibrahim Sahar,
Theus Andrea S.,
Kabboul Gabriella,
Do Katherine,
Bhamidipati Sai Raviteja,
Fischbach Jordan,
McCoy Kevin,
Zambrano Byron A.,
Zhang Jianyi,
Avazmohammadi Reza,
Mantalaris Athanasios,
Lindsey Brooks D.,
Frakes David,
Dasi Lakshmi Prasad,
Serpooshan Vahid,
BauserHeaton Holly
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202100968
Subject(s) - restenosis , anastomosis , shear stress , cardiology , stenosis , biomedical engineering , artery , pulmonary atresia , medicine , pulmonary artery , materials science , surgery , stent , composite material
Vascular atresia are often treated via transcatheter recanalization or surgical vascular anastomosis due to congenital malformations or coronary occlusions. The cellular response to vascular anastomosis or recanalization is, however, largely unknown and current techniques rely on restoration rather than optimization of flow into the atretic arteries. An improved understanding of cellular response post anastomosis may result in reduced restenosis. Here, an in vitro platform is used to model anastomosis in pulmonary arteries (PAs) and for procedural planning to reduce vascular restenosis. Bifurcated PAs are bioprinted within 3D hydrogel constructs to simulate a reestablished intervascular connection. The PA models are seeded with human endothelial cells and perfused at physiological flow rate to form endothelium. Particle image velocimetry and computational fluid dynamics modeling show close agreement in quantifying flow velocity and wall shear stress within the bioprinted arteries. These data are used to identify regions with greatest levels of shear stress alterations, prone to stenosis. Vascular geometry and flow hemodynamics significantly affect endothelial cell viability, proliferation, alignment, microcapillary formation, and metabolic bioprofiles. These integrated in vitro–in silico methods establish a unique platform to study complex cardiovascular diseases and can lead to direct clinical improvements in surgical planning for diseases of disturbed flow.