Premium
An Injectable Multifunctional Dual‐Phase Bead‐Reinforced Gelatin Matrix Permissive of Mesenchymal Stem Cell Infiltration for Musculoskeletal Soft Tissue Repair
Author(s) -
Du Dajiang,
Liu Zhen,
Niu Wanting,
Weng Ding,
Lim Teck Chuan,
Kurisawa Motoichi,
Spector Myron
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202100626
Subject(s) - gelatin , materials science , biomedical engineering , soft tissue , tissue engineering , phase (matter) , matrix (chemical analysis) , chemistry , composite material , surgery , medicine , biochemistry , organic chemistry
This study develops a novel strategy for regenerative therapy of musculoskeletal soft tissue defects using a dual‐phase multifunctional injectable gelatin‐hydroxyphenyl propionic acid (Gtn‐HPA) composite. The dual‐phase gel consists of stiff, degradation‐resistant, ≈2‐mm diameter spherical beads made from 8 wt% Gtn‐HPA in a 2 wt% Gtn‐HPA matrix. The results of a 3D migration assay show that both the cell number and migration distance in the dual‐phase gel system are comparable with the 2 wt% mono‐phase Gtn‐HPA, but notably significantly higher than for 8 wt% mono‐phase Gtn‐HPA (into which few cells migrated). The results also show that the dual phase gel system has degradation resistance and a prolonged growth factor release profile comparable with 8 wt% mono‐phase Gtn‐HPA. In addition, the compressive modulus of the 2 wt% dual‐phase gel system incorporating the 8 wt% bead phase is nearly four‐fold higher than the 2 wt% mono‐phase gel (5.3 ± 0.4 kPa versus 1.5 ± 0.06 kPa). This novel injectable dual‐phase Gtn‐HPA composite thus combines the advantages of low‐concentration Gtn‐HPA (cell migration) with high‐concentration Gtn‐HPA (stiffness, degradation resistance, slower chemical release kinetics) to facilitate effective reparative/regenerative processes in musculoskeletal soft tissue.