Premium
Light‐Regulated Angiogenesis via a Phototriggerable VEGF Peptidomimetic
Author(s) -
Nair Roshna V.,
Farrukh Aleeza,
del Campo Aránzazu
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202100488
Subject(s) - peptidomimetic , angiogenesis , vascular endothelial growth factor , self healing hydrogels , peptide , growth factor , endothelial stem cell , vascular endothelial growth factor a , chemistry , vegf receptors , biophysics , materials science , biology , biochemistry , cancer research , in vitro , receptor , organic chemistry
The application of growth factor based therapies in regenerative medicine is limited by the high cost, fast degradation kinetics, and the multiple functions of these molecules in the cell, which requires regulated delivery to minimize side effects. Here a photoactivatable peptidomimetic of the vascular endothelial growth factor (VEGF) that allows the light‐controlled presentation of angiogenic signals to endothelial cells embedded in hydrogel matrices is presented. A photoresponsive analog of the 15‐mer peptidomimetic Ac‐KLTWQELYQLKYKGI‐NH 2 (abbreviated P QK ) is prepared by introducing a 3‐(4,5‐dimethoxy‐2‐nitrophenyl)‐2‐butyl (DMNPB) photoremovable protecting group at the Trp4 residue. This modification inhibits the angiogenic potential of the peptide temporally. Light exposure of P QK modified hydrogels provide instructive cues to embedded endothelial cells and promote angiogenesis at the illuminated sites of the 3D culture, with the possibility of spatial control. P QK modified photoresponsive biomaterials offer an attractive approach for the dosed delivery and spatial control of pro‐angiogenic factors to support regulated vascular growth by just using light as an external trigger.