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All‐in‐One DNA Extraction Tube for Facilitated Real‐Time Detection of Infectious Pathogens
Author(s) -
Song Younseong,
Kim Yong Tae,
Choi Yunho,
Kim Hogi,
Yeom Min Hee,
Kim Yesol,
Lee Tae Jae,
Lee Kyoung G.,
Im Sung Gap
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202100430
Subject(s) - polymerase chain reaction , dna extraction , dna , tube (container) , pathogen , recombinase polymerase amplification , materials science , nanotechnology , computational biology , chromatography , biology , chemistry , genetics , gene , composite material
An “all‐in‐one tube” platform is developed, where the genetic analysis involving DNA extraction, amplification, and detection can be performed in a single tube. The all‐in‐one tube consists of a polymerase chain reaction (PCR) tube in which the inner surface is conformally modified with a tertiary‐amine‐containing polymer to generate a strong electrostatic interaction with DNA. The all‐in‐one tube provides high DNA capture efficiency exceeding 80% from Escherichia coli O157: H7 pathogen at a wide range of DNA amount from 0.003 to 3 ng. Indeed, the use of the surface‐functionalized PCR tube enables direct amplification and detection of the surface‐captured DNA without the modification of standard real‐time PCR instrument. Besides, this platform has sensitivity, selectivity, and reliability enough for accurate detection at the minimal infective dose of both gram‐positive and negative pathogens. The all‐in‐one tube enables the direct molecular diagnosis, substantially reducing the labor‐intensive pathogen detection steps while providing high compatibility with the currently established real‐time PCR instruments, and illustrates its on‐site applicability with convenience expandable to various genetic analyses including food safety testing, forensic analysis, and clinical diagnosis.