Premium
One‐Step All‐Aqueous Interfacial Assembly of Robust Membranes for Long‐Term Encapsulation and Culture of Adherent Stem/Stromal Cells
Author(s) -
Vilabril Sara,
Nadine Sara,
Neves Catarina M. S. S.,
Correia Clara R.,
Freire Mara G.,
Coutinho João A. P.,
Oliveira Mariana B.,
Mano João F.
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202100266
Subject(s) - microcarrier , cell encapsulation , mesenchymal stem cell , materials science , stromal cell , stem cell , self healing hydrogels , nanotechnology , polyelectrolyte , membrane , chemistry , chemical engineering , cell , polymer , microbiology and biotechnology , polymer chemistry , biochemistry , biology , engineering , composite material , cancer research
The therapeutic effectiveness and biological relevance of technologies based on adherent cells depend on platforms that enable long‐term culture in controlled environments. Liquid‐core capsules have been suggested as semipermeable moieties with spatial homogeneity due to the high mobility of all components in their core. The lack of cell‐adhesive sites in liquid‐core structures often hampers their use as platforms for stem cell‐based technologies for long‐term survival and cell‐directed self‐organization. Here, the one‐step fast formation of robust polymeric capsules formed by interfacial complexation of oppositely charged polyelectrolytes in an all‐aqueous environment, compatible with the simultaneous encapsulation of mesenchymal stem/stromal cells (MSCs) and microcarriers, is described. The adhesion of umbilical cord MSCs to polymeric microcarriers enables their aggregation and culture for more than 21 days in capsules prepared either manually by dropwise addition, or by scalable electrohydrodynamic atomization, generating robust and stable capsules. Cell aggregation and secretion overtime can be tailored by providing cells with static or dynamic (bioreactor) environments.