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Fast Stereolithography Printing of Large‐Scale Biocompatible Hydrogel Models
Author(s) -
Anandakrishnan Nanditha,
Ye Hang,
Guo Zipeng,
Chen Zhaowei,
Mentkowski Kyle I.,
Lang Jennifer K.,
Rajabian Nika,
Andreadis Stelios T.,
Ma Zhen,
Spernyak Joseph A.,
Lovell Jonathan F.,
Wang Depeng,
Xia Jun,
Zhou Chi,
Zhao Ruogang
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202002103
Subject(s) - stereolithography , 3d printing , self healing hydrogels , prepolymer , materials science , biocompatible material , fabrication , photopolymer , tissue engineering , biomedical engineering , nanotechnology , composite material , polymer , polymerization , engineering , polymer chemistry , medicine , alternative medicine , pathology , polyurethane
Large size cell‐laden hydrogel models hold great promise for tissue repair and organ transplantation, but their fabrication using 3D bioprinting is limited by the slow printing speed that can affect the part quality and the biological activity of the encapsulated cells. Here a fast hydrogel stereolithography printing (FLOAT) method is presented that allows the creation of a centimeter‐sized, multiscale solid hydrogel model within minutes. Through precisely controlling the photopolymerization condition, low suction force‐driven, high‐velocity flow of the hydrogel prepolymer is established that supports the continuous replenishment of the prepolymer solution below the curing part and the nonstop part growth. The rapid printing of centimeter‐sized hydrogel models using FLOAT is shown to significantly reduce the part deformation and cellular injury caused by the prolonged exposure to the environmental stresses in conventional 3D printing methods. Embedded vessel networks fabricated through multiscale printing allows media perfusion needed to maintain the high cellular viability and metabolic functions in the deep core of the large‐sized models. The endothelialization of this vessel network allows the establishment of barrier functions. Together, these studies demonstrate a rapid 3D hydrogel printing method and represent a first step toward the fabrication of large‐sized engineered tissue models.