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In Vivo Retention Quantification of Supramolecular Hydrogels Engineered for Cardiac Delivery
Author(s) -
Schotman Maaike J. G.,
Peters Marijn M. C.,
Krijger Gerard C.,
Adrichem Iris,
Roos Remmert,
Bemelmans John L. M.,
Pouderoijen Maarten J.,
Rutten Martin G. T. A.,
Neef Klaus,
Chamuleau Steven A. J.,
Dankers Patricia Y. W.
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202001987
Subject(s) - self healing hydrogels , in vivo , peg ratio , supramolecular chemistry , ethylene glycol , biomedical engineering , materials science , drug delivery , chemistry , moiety , biophysics , nanotechnology , polymer chemistry , stereochemistry , organic chemistry , medicine , biology , microbiology and biotechnology , finance , economics , crystal structure
Abstract Recent advances in the field of cardiac regeneration show great potential in the use of injectable hydrogels to reduce immediate flush‐out of injected factors, thereby increasing the effectiveness of the encapsulated drugs. To establish a relation between cardiac function and retention of the drug‐encapsulating hydrogel, a quantitative in vivo imaging method is required. Here, the supramolecular ureido‐pyrimidinone modified poly(ethylene glycol) (UPy‐PEG) material is developed into a bioactive hydrogel for radioactive imaging in a large animal model. A radioactive label is synthesized, being a ureido‐pyrimidinone moiety functionalized with a chelator (UPy‐DOTA) complexed with the radioactive isotope indium‐111 (UPy‐DOTA‐ 111 In) that is mixed with the hydrogel. Additionally, bioactive and adhesive properties of the UPy‐PEG hydrogel are increased by supramolecular introduction of a UPy‐functionalized recombinant collagen type 1‐based material (UPy‐PEG‐RCPhC1). This method enables in vivo tracking of the nonbioactive and bioactive supramolecular hydrogels and quantification of hydrogel retention in a porcine heart. In a small pilot, cardiac retention values of 8% for UPy‐PEG and 16% for UPy‐PEG‐RCPhC1 hydrogel are observed 4 h postinjection. This work highlights the importance of retention quantification of hydrogels in vivo, where elucidation of hydrogel quantity at the target site is proposed to strongly influence efficacy of the intended therapy.