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Nanocellulose‐Reinforced Hydroxyapatite Nanobelt Membrane as a Stem Cell Multi‐Lineage Differentiation Platform for Biomimetic Construction of Bioactive 3D Osteoid Tissue In Vitro
Author(s) -
Liu Feng,
Wei Benjie,
Xu Xiaoying,
Ma Baojin,
Zhang Shan,
Duan Jiazhi,
Kong Ying,
Yang Hongru,
Sang Yuanhua,
Wang Shuhua,
Tang Wei,
Liu Chao,
Liu Hong
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202001851
Subject(s) - mesenchymal stem cell , osteoid , stem cell , materials science , microbiology and biotechnology , biomedical engineering , nanocellulose , cellular differentiation , scaffold , tissue engineering , bone tissue , anatomy , chemistry , biology , medicine , biochemistry , cellulose , gene
Abstract Severe bone defects, especially accompanied by vascular and peripheral nerve injuries, remain a massive challenge. Most studies related to bone tissue engineering have focused on osteogenic differentiation of mesenchymal stem cells (MSCs), and ignored the formation of blood vessels and nerves in the newly generated bone owing to the lack of proper materials and methodology for tuning stem cells differentiated into osteogenic, neuronal, and endothelial cells (ECs) in the same scaffold system. Herein, a nanocellulose‐reinforced hybrid membrane with good mechanical properties and control over biodegradation by assembling ultralong hydroxyapatite nanobelts in a bacterial nanocellulose hydrogel is designed and synthesized. Osteogenic, neuronal cells are successfully differentiated on this hybrid membrane. Based on the multi‐lineage differentiation property of the membrane, a bioactive 3D osteoid tissue (osteogenic, neural, and ECs) is mimetically constructed in vitro using layer‐by‐layer culture and integration. The bone regeneration ability of the as‐prepared bioactive osteoid tissue is assessed in vivo via heterotopic osteogenesis experiments for eight weeks. The rapid new bone growth and formation of blood capillaries and nerve fibers prove that the hybrid membrane can be universally applied as a stem cell multi‐lineage differentiation platform, which has significant applications in bone tissue engineering.