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Patient‐Specific 3D Bioprinted Models of Developing Human Heart
Author(s) -
Cetnar Alexander D.,
Tomov Martin L.,
Ning Liqun,
Jing Bowen,
Theus Andrea S.,
Kumar Akaash,
Wijntjes Amanda N.,
Bhamidipati Sai Raviteja,
Do Katherine Pham,
Mantalaris Athanasios,
Oshinski John N.,
Avazmohammadi Reza,
Lindsey Brooks D.,
BauserHeaton Holly D.,
Serpooshan Vahid
Publication year - 2021
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.202001169
Subject(s) - 3d bioprinting , heart development , human heart , biomedical engineering , embryonic heart , ventricle , computer science , magnetic resonance imaging , embryonic stem cell , neuroscience , biology , tissue engineering , cardiology , medicine , radiology , biochemistry , gene
The heart is the first organ to develop in the human embryo through a series of complex chronological processes, many of which critically rely on the interplay between cells and the dynamic microenvironment. Tight spatiotemporal regulation of these interactions is key in heart development and diseases. Due to suboptimal experimental models, however, little is known about the role of microenvironmental cues in the heart development. This study investigates the use of 3D bioprinting and perfusion bioreactor technologies to create bioartificial constructs that can serve as high‐fidelity models of the developing human heart. Bioprinted hydrogel‐based, anatomically accurate models of the human embryonic heart tube (e‐HT, day 22) and fetal left ventricle (f‐LV, week 33) are perfused and analyzed both computationally and experimentally using ultrasound and magnetic resonance imaging. Results demonstrate comparable flow hemodynamic patterns within the 3D space. We demonstrate endothelial cell growth and function within the bioprinted e‐HT and f‐LV constructs, which varied significantly in varying cardiac geometries and flow. This study introduces the first generation of anatomically accurate, 3D functional models of developing human heart. This platform enables precise tuning of microenvironmental factors, such as flow and geometry, thus allowing the study of normal developmental processes and underlying diseases.