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Protein Delivery: Intracellular Delivery of His‐Tagged Genome‐Editing Proteins Enabled by Nitrilotriacetic Acid–Containing Lipidoid Nanoparticles (Adv. Healthcare Mater. 6/2019)
Author(s) -
Li Yamin,
Li Alice Chukun,
Xu Qiaobing
Publication year - 2019
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201970021
Subject(s) - nitrilotriacetic acid , nanoparticle , cas9 , genome editing , cationic polymerization , intracellular , ribonucleoprotein , chemistry , crispr , nanotechnology , biochemistry , materials science , rna , polymer chemistry , gene , organic chemistry , chelation
In article number 1800996 , Qiaobing Xu and co‐workers report a new type of non‐cationic lipidoid nanoparticle for His‐tagged protein delivery. Active lipidoids are synthesized by conjugating nitrilotriacetic acid with hydrophobic tails and nanoparticles are formulated in the presence of helper lipids through self‐assembly. The newly developed lipidoid nanoparticles are capable of delivering genome‐editing proteins (Cre recombinase and CRISPR/Cas9 ribonucleoprotein) into mammalian cells.