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3D Bioprinting of Oxygenated Cell‐Laden Gelatin Methacryloyl Constructs
Author(s) -
Erdem Ahmet,
Darabi Mohammad Ali,
Nasiri Rohollah,
Sangabathuni Sivakoti,
Ertas Yavuz Nuri,
Alem Halima,
Hosseini Vahid,
Shamloo Amir,
Nasr Ali S.,
Ahadian Samad,
Dokmeci Mehmet R.,
Khademhosseini Ali,
Ashammakhi Nureddin
Publication year - 2020
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201901794
Subject(s) - 3d bioprinting , gelatin , viability assay , chemistry , self healing hydrogels , transplantation , cell survival , biofabrication , tissue engineering , biomedical engineering , cell , biochemistry , polymer chemistry , in vitro , surgery , medicine
Cell survival during the early stages of transplantation and before new blood vessels formation is a major challenge in translational applications of 3D bioprinted tissues. Supplementing oxygen (O 2 ) to transplanted cells via an O 2 generating source such as calcium peroxide (CPO) is an attractive approach to ensure cell viability. Calcium peroxide also produces calcium hydroxide that reduces the viscosity of bioinks, which is a limiting factor for bioprinting. Therefore, adapting this solution into 3D bioprinting is of significant importance. In this study, a gelatin methacryloyl (GelMA) bioink that is optimized in terms of pH and viscosity is developed. The improved rheological properties lead to the production of a robust bioink suitable for 3D bioprinting and controlled O 2 release. In addition, O 2 release, bioprinting conditions, and mechanical performance of hydrogels having different CPO concentrations are characterized. As a proof of concept study, fibroblasts and cardiomyocytes are bioprinted using CPO containing GelMA bioink. Viability and metabolic activity of printed cells are checked after 7 days of culture under hypoxic condition. The results show that the addition of CPO improves the metabolic activity and viability of cells in bioprinted constructs under hypoxic condition.