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Supramolecular Nanofibers for Encapsulation and In Situ Differentiation of Neural Stem Cells
Author(s) -
Zhao Hao,
Xu Jingwen,
Peng Ke,
Fu Xuancheng,
Zhang Endong,
Lv Fengting,
Liu Libing,
Zhang Na,
Wang Yilin,
Wang Shu,
Gu Qi
Publication year - 2020
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201901295
Subject(s) - nanofiber , materials science , in situ , neural stem cell , tissue engineering , biocompatibility , nanotechnology , chromatin , supramolecular chemistry , biophysics , stem cell , microbiology and biotechnology , chemistry , dna , biology , biochemistry , molecule , genetics , organic chemistry , metallurgy
Design and fabrication of fibrous materials by natural biological macromolecules in light of biomimetics to achieve spatially cellular arrangements are highly desirable in tissue engineering. Herein, chromatin‐inspired supramolecular fibers formed through the interfacial polyelectrolyte complexation (IPC) process by DNA and histone proteins for encapsulation and in situ differentiation of murine brain‐derived neural stem cells (NSCs) are reported. High cell viability of encapsulated NSCs demonstrates the excellent biocompatibility of fibers as 3D scaffolds. Moreover, a cell‐adhesive peptide (K 6 ‐PEG‐RGD) is introduced into fibers by electrostatic interaction to improve NSCs encapsulation efficiency and prevent them from migrating out of fibers for enhanced spatially cellular arrangement. In situ differentiation of NSCs into oligodendrocytes within fibers is revealed by immunocytochemical staining assay. Due to the robust abilities to encapsulate and in situ differentiate NSCs, these chromatin‐inspired supramolecular fibers show great potential in neural system‐related tissue.