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Extracellular Trap‐Mimicking DNA‐Histone Mesostructures Synergistically Activate Dendritic Cells
Author(s) -
Weerappuli Priyan D.,
Louttit Cameron,
Kojima Taisuke,
Brennan Luke,
Yalavarthi Srilakshmi,
Xu Yao,
Ochyl Lukasz J.,
Maeda Midori L.,
Kim Hong Sun,
Knight Jason S.,
Takayama Shuichi,
Moon James J.
Publication year - 2019
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201900926
Subject(s) - histone , microbiology and biotechnology , immune system , neutrophil extracellular traps , dna , extracellular , dna methylation , biology , chemistry , inflammation , genetics , immunology , gene , gene expression
Abstract Extracellular traps (ETs), such as neutrophil extracellular traps, are a physical mesh deployed by immune cells to entrap and constrain pathogens. ETs are immunogenic structures composed of DNA, histones, and an array of variable protein and peptide components. While much attention has been paid to the multifaceted function of these structures, mechanistic studies of ETs remain challenging due to their heterogeneity and complexity. Here, a novel DNA‐histone mesostructure (DHM) formed by complexation of DNA and histones into a fibrous mesh is reported. DHMs mirror the DNA‐histone structural frame of ETs and offer a facile platform for cell culture studies. It is shown that DHMs are potent activators of dendritic cells and identify both the methylation state of DHMs and physical interaction between dendritic cells and DHMs as key tuning switches for immune stimulation. Overall, the DHM platform provides a new opportunity to study the role of ETs in immune activation and pathophysiology.