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Intracellular Delivery of His‐Tagged Genome‐Editing Proteins Enabled by Nitrilotriacetic Acid–Containing Lipidoid Nanoparticles
Author(s) -
Li Yamin,
Li Alice Chukun,
Xu Qiaobing
Publication year - 2019
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201800996
Subject(s) - nitrilotriacetic acid , green fluorescent protein , chemistry , intracellular , cas9 , genome editing , nanoparticle , ribonucleoprotein , peptide , polyethylene glycol , biochemistry , amino acid , crispr , nanotechnology , materials science , gene , rna , organic chemistry , chelation
Protein‐ and peptide‐based therapeutics with high tolerance and specificity along with low off‐target effects and genetic risks have attracted tremendous attention over the last three decades. Herein, a new type of noncationic lipidoid nanoparticle (LNP) is reported for His‐tagged protein delivery. Active lipidoids are synthesized by conjugating a nitrilotriacetic acid group with hydrophobic tails (EC16, O16B, and O17O) and nanoparticles are formulated in the presence of nickel ions and helper lipids (cholesterol, 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphoethanolamine, and 1,2‐distearoyl‐ sn ‐glycero‐3‐phosphoethanolamine‐ N ‐[methoxy(polyethylene glycol)‐2000]). It is demonstrated that the newly developed LNPs are capable of delivering various His‐tagged proteins including green fluorescent protein (GFP), (−30)GFP‐Cre recombinase, and CRISPR/Cas9 ribonucleoprotein into mammalian cells.