Premium
A Photo‐Crosslinkable Kidney ECM‐Derived Bioink Accelerates Renal Tissue Formation
Author(s) -
Ali Mohamed,
PR Anil Kumar,
Yoo James J.,
Zahran Faten,
Atala Anthony,
Lee Sang Jin
Publication year - 2019
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201800992
Subject(s) - decellularization , 3d bioprinting , extracellular matrix , tissue engineering , self healing hydrogels , biofabrication , materials science , biomedical engineering , kidney , nanotechnology , microbiology and biotechnology , biology , medicine , endocrinology , polymer chemistry
3D bioprinting strategies in tissue engineering aim to fabricate clinically applicable tissue constructs that can replace the damaged or diseased tissues and organs. One of the main prerequisites in 3D bioprinting is finding an appropriate bioink that provides a tissue‐specific microenvironment supporting the cellular growth and maturation. In this respect, decellularized extracellular matrix (dECM)‐derived hydrogels have been considered as bioinks for the cell‐based bioprinting due to their capability to inherit the intrinsic cues from native ECM. Herein, a photo‐crosslinkable kidney ECM‐derived bioink (KdECMMA) is developed that could provide a kidney‐specific microenvironment for renal tissue bioprinting. Porcine whole kidneys are decellularized through a perfusion method, dissolved in an acid solution, and chemically modified by methacrylation. A KdECMMA‐based bioink is formulated and evaluated for rheological properties and printability for the printing process. The results show that the bioprinted human kidney cells in the KdECMMA bioink are highly viable and mature with time. Moreover, the bioprinted renal constructs exhibit the structural and functional characteristics of the native renal tissue. The potential of the tissue‐specific ECM‐derived bioink is demonstrated for cell‐based bioprinting that could enhance the cellular maturation and eventually tissue formation.