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Amnion‐Analogous Medical Device for Fetal Membrane Healing: A Preclinical Long‐Term Study
Author(s) -
Lee Jae Yeon,
Kim Hyeonji,
Ha DongHeon,
Shin Jong Chul,
Kim Ahyoung,
Ko Hyun Sun,
Cho DongWoo
Publication year - 2018
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201800673
Subject(s) - amnion , decellularization , biocompatible material , fetus , fetal membrane , fetoscopy , biomedical engineering , amniotic fluid , medicine , surgery , pregnancy , tissue engineering , prenatal diagnosis , biology , genetics , placenta
Although recent invasive fetal surgeries have improved fetal outcomes, fetal membrane rupture remains a major complication, leading to premature delivery, thus undermining the complete benefits of such procedures. A biocompatible amnion‐analogous medical device (AMED) consisting of polycaprolactone framework and decellularized amniotic membrane (dAM)–derived hydrogel for restoration of amniotic membrane defect is developed using 3D printing technology. Its efficacy on healing iatrogenic fetal membrane defects in vitro is evaluated, showing that the dAM gel contains migratory and proliferative properties. The fetoscope feasibility of the developed AMED is assessed using a pregnant swine model. All animals had successfully recovered from anesthesia and the fetoscopic procedure and maintained a healthy condition until the end of the pregnancy. AMED exhibits superior surgical handling characteristics and is easy to manufacture, nonimmunogenic, biocompatible, and suitable for storage and transport for off‐the‐shelf use; hence, it can be used in successfully sealing defect sites, thus improving the preservation of the amniotic fluid, which in turn improves fetal survival and development.