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3D Interconnected Mesoporous Alumina with Loaded Hemoglobin as a Highly Active Electrochemical Biosensor for H 2 O 2
Author(s) -
Yang Xuanyu,
Cheng Xiaowei,
Song Hongyuan,
Ma Junhao,
Pan Panpan,
Elzatahry Ahmed A.,
Su Jiacan,
Deng Yonghui
Publication year - 2018
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201800149
Subject(s) - mesoporous material , materials science , biosensor , adsorption , biomolecule , detection limit , electrocatalyst , chemical engineering , copolymer , hydrogen peroxide , electrochemistry , nuclear chemistry , nanotechnology , chemistry , electrode , catalysis , organic chemistry , chromatography , polymer , composite material , engineering
Alumina is one of the most common and stable metal oxides in nature, which has been developed as a novel adsorbent in enrichment of biomolecules due to its excellent affinity to phosphor or amino groups. In this study, ordered mesoporous alumina (OMA) with interconnected mesopores and surface acidic property is synthesized through a solvent evaporation induced co‐assembly process using poly(ethylene oxide)‐ b ‐polystyrene (PEO‐ b ‐PS) diblock copolymer as a template and aluminium acetylacetonate (Al(acac) 3 ) as the aluminium source. The pore size (12.1–19.7 nm), pore window size (3.5–9.0 nm) and surface acidity (0.092–0.165 mmol g −1 ) can be precisely adjusted. The highly porous structure endows the OMA materials with high hemoglobin (Hb) immobilization capacity (170 mg g −1 ). The obtained Hb@OMA composite is used as an electrocatalyst of biosensor for convienet and fast detection of hydrogen peroxide (H 2 O 2 ) with a low H 2 O 2 detection limit of 1.7 × 10 −8 m and a wide linear range of 2.5 × 10 −8 to 5.0 × 10 −5 m . Moreover, the Hb@OMA sensors show a good performance in real time detection of H 2 O 2 released from Homo sapiens bone osteosarcoma, indicating their potential application in complex biological processes.