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A Novel, Well‐Resolved Direct Laser Bioprinting System for Rapid Cell Encapsulation and Microwell Fabrication
Author(s) -
Wang Zongjie,
Jin Xian,
Tian Zhenlin,
Menard Frederic,
Holzman Jonathan F.,
Kim Keekyoung
Publication year - 2018
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201701249
Subject(s) - materials science , fabrication , laser , nanotechnology , seeding , photoinitiator , optics , composite material , medicine , physics , alternative medicine , monomer , engineering , pathology , aerospace engineering , polymer
A direct laser bioprinting (DLBP) system is introduced in this work. The DLBP system applies visible‐laser‐induced photo‐crosslinking at a wavelength of 405 nm using the photoinitiator VA‐086. It is shown that such a system can fabricate vertical structures with fine features (less than 50 µm) and high cell viability (greater than 95%). Experimental characterizations and theoretical simulations are presented, and good agreement is seen between the experiments and theory. The DLBP system is applied to the fabrication of (1) cell‐laden hydrogel microgrids, (2) hydrogel microwells, as well as a test of (3) cell encapsulation, and (4) cell seeding. The DLBP system is found to be a promising tool for bioprinting.