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Heparinization of Beta Tricalcium Phosphate: Osteo‐immunomodulatory Effects
Author(s) -
DiezEscudero Anna,
Espanol Montserrat,
Bonany Mar,
Lu Xi,
Persson Cecilia,
Ginebra MariaPau
Publication year - 2018
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201700867
Subject(s) - proinflammatory cytokine , mesenchymal stem cell , microbiology and biotechnology , immune system , downregulation and upregulation , tumor necrosis factor alpha , macrophage , calcium , inflammation , cell adhesion , adhesion , chemistry , materials science , cell , immunology , biology , biochemistry , in vitro , organic chemistry , gene
Immune cells play a vital role in regulating bone dynamics. This has boosted the interest in developing biomaterials that can modulate both the immune and skeletal systems. In this study, calcium phosphates discs (i.e., beta‐tricalcium phosphate, β‐TCP) are functionalized with heparin to investigate the effects on immune and stem cell responses. The results show that the functionalized surfaces downregulate the release of hydrogen peroxide and proinflammatory cytokines (tumor necrosis factor alpha and interleukin 1 beta) from human monocytes and neutrophils, compared to nonfunctionalized discs. The macrophages show both elongated and round shapes on the two ceramic substrates, but the morphology of cells on heparinized β‐TCP tends toward a higher elongation after 72 h. The heparinized substrates support rat mesenchymal stem cell (MSC) adhesion and proliferation, and anticipate the differentiation toward the osteoblastic lineage as compared to β‐TCP and control. The coupling between the inflammatory response and osteogenesis is assessed by culturing MSCs with the macrophage supernatants. The downregulation of inflammation in contact with the heparinized substrates induces higher expression of bone‐related markers by MSCs.