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Glycan Stimulation Enables Purification of Prostate Cancer Circulating Tumor Cells on PEDOT NanoVelcro Chips for RNA Biomarker Detection
Author(s) -
Shen MoYuan,
Chen JieFu,
Luo ChunHao,
Lee Sangjun,
Li ChengHsuan,
Yang YungLing,
Tsai YuHan,
Ho BoCheng,
Bao LiRong,
Lee TienJung,
Jan Yu Jen,
Zhu YaZhen,
Cheng Shirley,
Feng Felix Y.,
Chen Peilin,
Hou Shuang,
Agopian Vatche,
Hsiao YuSheng,
Tseng HsianRong,
Posadas Edwin M.,
Yu Hsiaohua
Publication year - 2018
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201700701
Subject(s) - pedot:pss , circulating tumor cell , prostate cancer , glycan , rna , cancer research , cancer , chemistry , materials science , nanotechnology , medicine , biochemistry , layer (electronics) , metastasis , gene , glycoprotein
A glycan‐stimulated and poly(3,4‐ethylene‐dioxythiophene)s (PEDOT)‐based nanomaterial platform is fabricated to purify circulating tumor cells (CTCs) from blood samples of prostate cancer (PCa) patients. This new platform, phenylboronic acid (PBA)‐grafted PEDOT NanoVelcro, combines the 3D PEDOT nanosubstrate, which greatly enhances CTC capturing efficiency, with a poly(EDOT‐PBA‐ co ‐EDOT‐EG3) interfacial layer, which not only provides high specificity for CTC capture upon antibody conjugation but also enables competitive binding of sorbitol to gently release the captured cells. CTCs purified by this PEDOT NanoVelcro chip provide well‐preserved RNA transcripts for the analysis of the expression level of several PCa‐specific RNA biomarkers, which may provide clinical insights into the disease.