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Surface‐Modified Mesh Filter for Direct Nucleic Acid Extraction and its Application to Gene Expression Analysis
Author(s) -
You Jae Bem,
Kim Yong Tae,
Lee Kyoung G.,
Choi Yunho,
Choi Seongkyun,
Kim Chi Hyun,
Kim Kyung Hoon,
Chang Sung Jin,
Lee Tae Jae,
Lee Seok Jae,
Im Sung Gap
Publication year - 2017
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201700642
Subject(s) - lysis , zeta potential , nucleic acid , cationic polymerization , surface charge , filter (signal processing) , materials science , fluorescence , surface modification , nanotechnology , chemistry , nanoparticle , computer science , biochemistry , polymer chemistry , physics , quantum mechanics , computer vision
Rapid and convenient isolation of nucleic acids (NAs) from cell lysate plays a key role for onsite gene expression analysis. Here, this study achieves one‐step and efficient capture of NA directly from cell lysate by developing a cationic surface‐modified mesh filter (SMF). By depositing cationic polymer via vapor‐phase deposition process, strong charge interaction is introduced on the surface of the SMF to capture the negatively charged NAs. The NA capturing capability of SMF is confirmed by X‐ray photoelectron spectroscopy, fluorescent microscopy, and zeta potential measurement. In addition, the genomic DNAs of Escherichia Coli O157:H7 can be extracted by the SMF from artificially infected food, and fluorescent signal is observed on the surface of SMF after amplification of target gene. The proposed SMF is able to provide a more simplified, convenient, and fast extraction method and can be applied to the fields of food safety testing, clinical diagnosis, or environmental pollutant monitoring.