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Engineered Paper‐Based Cell Culture Platforms
Author(s) -
Lantigua Darlin,
Kelly Yan Ni,
Unal Baris,
CamciUnal Gulden
Publication year - 2017
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201700619
Subject(s) - multicellular organism , modular design , tissue engineering , biocompatible material , nanotechnology , computer science , scaffold , 3d cell culture , cell culture , materials science , cell , biomedical engineering , chemistry , biology , engineering , biochemistry , genetics , database , operating system
Abstract Paper is used in various applications in biomedical research including diagnostics, separations, and cell cultures. Paper can be conveniently engineered due to its tunable and flexible nature, and is amenable to high‐throughput sample preparation and analysis. Paper‐based platforms are used to culture primary cells, tumor cells, patient biopsies, stem cells, fibroblasts, osteoblasts, immune cells, bacteria, fungi, and plant cells. These platforms are compatible with standard analytical assays that are typically used to monitor cell behavior. Due to its thickness and porous nature, there are no mass transport limitations to/from the cells in paper scaffolds. It is possible to pattern paper in different scales (micrometer to centimeter), generate modular configurations in 3D, fabricate multicellular and compartmentalized tissue mimetics for clinical applications, and recover cells from the scaffolds for further analysis. 3D paper constructs can provide physiologically relevant tissue models for personalized medicine. Layer‐by layer strategies to assemble tissue‐like structures from low‐cost and biocompatible paper‐based materials offer unique opportunities that include understanding fundamental biology, developing disease models, and assembling different tissues for organ‐on‐paper applications. Paper‐based platforms can also be used for origami‐inspired tissue engineering. This work provides an overview of recent progress in engineered paper‐based biomaterials and platforms to culture and analyze cells.