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Spatially Assembled Bilayer Cell Sheets of Stem Cells and Endothelial Cells Using Thermosensitive Hydrogels for Therapeutic Angiogenesis
Author(s) -
Jun Indong,
Ahmad Taufiq,
Bak Seongwoo,
Lee JoongYup,
Kim Eun Mi,
Lee Jinkyu,
Lee Yu Bin,
Jeong Hongsoo,
Jeon Hojeong,
Shin Heungsoo
Publication year - 2017
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201601340
Subject(s) - self healing hydrogels , mesenchymal stem cell , in vivo , microbiology and biotechnology , angiogenesis , transplantation , materials science , bilayer , endothelial stem cell , cell , tissue engineering , fibronectin , stem cell , in vitro , biomedical engineering , chemistry , extracellular matrix , cancer research , medicine , biology , biochemistry , surgery , membrane , polymer chemistry
Although the coculture of multiple cell types has been widely employed in regenerative medicine, in vivo transplantation of cocultured cells while maintaining the hierarchical structure remains challenging. Here, a spatially assembled bilayer cell sheet of human mesenchymal stem cells and human umbilical vein endothelial cells on a thermally expandable hydrogel containing fibronectin is prepared and its effect on in vitro proangiogenic functions and in vivo ischemic injury is investigated. The expansion of hydrogels in response to a temperature change from 37 to 4 °C allows rapid harvest and delivery of the bilayer cell sheet to two different targets (an in vitro model glass surface and in vivo tissue). The in vitro study confirms that the bilayer sheet significantly increases proangiogenic functions such as the release of nitric oxide and expression of vascular endothelial cell genes. In addition, transplantation of the cell sheet from the hydrogels into a hindlimb ischemia mice model demonstrates significant retardation of necrosis particularly in the group transplated with the bilayer sheet. Collectively, the bilayer cell sheet is readily transferrable from the thermally expandable hydrogel and represents an alternative approach for recovery from ischemic injury, potentially via improved cell–cell communication.