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Mesenchymal Stem Cell Magnetization: Magnetic Multilayer Microcapsule Uptake, Toxicity, Impact on Functional Properties, and Perspectives for Magnetic Delivery
Author(s) -
Lepik Kirill V.,
Muslimov Albert R.,
Timin Alexander S.,
Sergeev Vladislav S.,
Romanyuk Dmitry S.,
Moiseev Ivan S.,
Popova Elena V.,
Radchenko Igor L.,
Vilesov Alexander D.,
Galibin Oleg V.,
Sukhorukov Gleb B.,
Afanasyev Boris V.
Publication year - 2016
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201600843
Subject(s) - mesenchymal stem cell , internalization , microbiology and biotechnology , cell , materials science , capsule , nanotechnology , cell culture , cell therapy , stem cell , magnetic nanoparticles , biophysics , chemistry , biology , nanoparticle , biochemistry , botany , genetics
Mesenchymal stem cells (MSCs) are widely used in cell therapy due to their convenience, multiline differentiation potential, reproducible protocols, and biological properties. The potential of MSCs to impregnate magnetic microcapsules and their possible influence on cell function and ability to response to magnetic field have been explored. Interestingly, the cells suspended in media show much higher ability in internalization of microcapsules, then MSCs adhere into the surface. There is no significant effect of microcapsules on cell toxicity compared with other cell line‐capsule internalization reported in literature. Due to internalization of magnetic capsules by the cells, such cell engineering platform is responsive to external magnetic field, which allows to manipulate MSC migration. Magnetically sorted MSCs are capable to differentiation as confirmed by their conversion to adipogenic and osteogenic cells using standard protocols. There is a minor effect of capsule internalization on cell adhesion, though MSCs are still able to form spheroid made by dozen of thousand MSCs. This work demonstrates the potential of use of microcapsule impregnated MSCs to carry internalized micron‐sized vesicles and being navigated with external magnetic signaling.