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Hemoglobin‐Conjugated Gelatin Microsphere as a Smart Oxygen Releasing Biomaterial
Author(s) -
Paciello Antonio,
Amalfitano Giuseppe,
Garziano Alessandro,
Urciuolo Francesco,
Netti Paolo A.
Publication year - 2016
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201600559
Subject(s) - gelatin , fourier transform infrared spectroscopy , conjugated system , materials science , biomaterial , scanning electron microscope , hemoglobin , amide , oxygen , chemical engineering , analytical chemistry (journal) , chemistry , nanotechnology , polymer , chromatography , organic chemistry , engineering , composite material
In this study, a novel micrometric biomaterial acting as a cyclic oxygen releasing system is designed. Human hemoglobin (Hb) is conjugated to the surface of gelatin microspheres (GM) to produce gelatin hemoglobin oxygen depot (G‐HbOD). G‐HbOD is obtained by means of two different conjugation strategies. The degree of conjugation of GM surfaces in terms of free amino groups by using HPLC is first evaluated. By following the strategy A (G‐HbOD_A), Hb is conjugated to GM by means of the formation of a polyurethane linker. In the strategy B (G‐HbOD_B) the conjugation occurs via amide bound formation. Physical and morphological differences between G‐HbOD_A and G‐HbOD_B are investigated by means of Fourier Transform Infrared Spectroscopy (FTIR), Inductively Coupled Plasma Optical Emission Spectroscopy (ICP‐OES), Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Differences in oxygen uptake/release kinetics are found depending on the conjugation strategy and it is proved that G‐HbOD works under repeated cycles in microfluidic chip. Moreover, G‐HbOD is also able to work as oxygen depot in the early stages of 3D cell cultures.