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3D Cell Printing of Functional Skeletal Muscle Constructs Using Skeletal Muscle‐Derived Bioink
Author(s) -
Choi YeongJin,
Kim Taek Gyoung,
Jeong Jonghyeon,
Yi HeeGyeong,
Park Ji Won,
Hwang Woonbong,
Cho DongWoo
Publication year - 2016
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201600483
Subject(s) - decellularization , skeletal muscle , extracellular matrix , myocyte , microbiology and biotechnology , tissue engineering , cell , muscle tissue , agrin , biomedical engineering , chemistry , materials science , anatomy , biology , medicine , acetylcholine receptor , receptor , biochemistry
Engineered skeletal muscle tissues that mimic the structure and function of native muscle have been considered as an alternative strategy for the treatment of various muscular diseases and injuries. Here, it is demonstrated that 3D cell‐printing of decellularized skeletal muscle extracellular matrix (mdECM)‐based bioink facilitates the fabrication of functional skeletal muscle constructs. The cellular alignment and the shape of the tissue constructs are controlled by 3D cell‐printing technology. mdECM bioink provides the 3D cell‐printed muscle constructs with a myogenic environment that supports high viability and contractility as well as myotube formation, differentiation, and maturation. More interestingly, the preservation of agrin is confirmed in the mdECM, and significant increases in the formation of acetylcholine receptor clusters are exhibited in the 3D cell‐printed muscle constructs. In conclusion, mdECM bioink and 3D cell‐printing technology facilitate the mimicking of both the structural and functional properties of native muscle and hold great promise for producing clinically relevant engineered muscle for the treatment of muscular injuries.