Premium
Multicolor Immunofluorescent Imaging of Complex Cellular Mixtures on Micropallet Arrays Enables the Identification of Single Cells of Defined Phenotype
Author(s) -
Westerhof Trisha M.,
Li GuannPyng,
Bachman Mark,
Nelson Edward L.
Publication year - 2016
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201500859
Subject(s) - phenotype , confocal , confocal microscopy , identification (biology) , cell , microbiology and biotechnology , materials science , immunofluorescence , biophysics , computational biology , biology , optics , genetics , gene , antibody , physics , botany
A Micropallet‐Array‐based strategy allowing the identification of cells of defined phenotype in complex mixtures, such as would be obtained from a tissue biopsy, is presented. Following the distribution of single adherent cells from the mixture on individual pedestals, termed “micropallets”, immunofluorescent confocal imaging is applied to interrogate the expression of five cell surface molecules.