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Effect of Shape, Size, and Aspect Ratio on Nanoparticle Penetration and Distribution inside Solid Tissues Using 3D Spheroid Models
Author(s) -
Agarwal Rachit,
Jurney Patrick,
Raythatha Mansi,
Singh Vikramjit,
Sreenivasan Sidlgata. V.,
Shi Li,
Roy Krishnendu
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201500441
Subject(s) - penetration (warfare) , spheroid , materials science , nanoparticle , nanotechnology , aspect ratio (aeronautics) , nanoscopic scale , nanocarriers , nanomedicine , drug delivery , penetration depth , nanorod , biomedical engineering , composite material , optics , chemistry , medicine , biochemistry , physics , operations research , engineering , in vitro
Efficient penetration and uniform distribution of nanoparticles (NPs) inside solid tissues and tumors is paramount to their therapeutic and diagnostic success. While many studies have reported the effect of NP size and charge on intratissue distribution, role of shape, and aspect ratio on NP transport inside solid tissues remain unclear. Here experimental and theoretical studies are reported on how nanoscale geometry of Jet and Flash Imprint Lithography‐fabricated, polyethylene‐glycol‐based anionic nanohydrogels affect their penetration and distribution inside 3D spheroids, a model representing the intervascular region of solid, tumor‐like tissues. Unexpectedly, low aspect ratio cylindrical NPs (H/D ≈0.3; disk‐like particles, 100 nm height, and 325 nm diameter) show maximal intratissue delivery (>50% increase in total cargo delivered) and more uniform penetration compared to nanorods or smaller NPs of the same shape. This is in contrast to spherical NPs where smaller NP size resulted in deeper, more uniform penetration. Our results provide fundamental new knowledge on NP transport inside solid tissues and further establish shape and aspect ratio as important design parameters in developing more efficient, better penetrating, nanocarriers for drug, or contrast‐agent delivery.