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Engineering Cell‐Compatible Paper Chips for Cell Culturing, Drug Screening, and Mass Spectrometric Sensing
Author(s) -
Chen Qiushui,
He Ziyi,
Liu Wu,
Lin Xuexia,
Wu Jing,
Li Haifang,
Lin JinMing
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201500383
Subject(s) - cell , cell adhesion , cell growth , materials science , nanotechnology , cell culture , tissue engineering , in situ , chemistry , biomedical engineering , biology , biochemistry , medicine , organic chemistry , genetics
Paper‐supported cell culture is an unprecedented development for advanced bioassays. This study reports a strategy for in vitro engineering of cell‐compatible paper chips that allow for adherent cell culture, quantitative assessment of drug efficiency, and label‐free sensing of intracellular molecules via paper spray mass spectrometry. The polycarbonate paper is employed as an excellent alternative bioscaffold for cell distribution, adhesion, and growth, as well as allowing for fluorescence imaging without light scattering. The cell‐cultured paper chips are thus amenable to fabricate 3D tissue construction and cocultures by flexible deformation, stacks and assembly by layers of cells. As a result, the successful development of cell‐compatible paper chips subsequently offers a uniquely flexible approach for in situ sensing of live cell components by paper spray mass spectrometry, allowing profiling the cellular lipids and quantitative measurement of drug metabolism with minimum sample pretreatment. Consequently, the developed paper chips for adherent cell culture are inexpensive for one‐time use, compatible with high throughputs, and amenable to label‐free and rapid analysis.