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A New Approach for Fabricating Collagen/ECM‐Based Bioinks Using Preosteoblasts and Human Adipose Stem Cells
Author(s) -
Lee Hyeong Jin,
Kim Yong Bok,
Ahn Seung Hyun,
Lee JiSeon,
Jang Chul Ho,
Yoon Hyeon,
Chun Wook,
Kim Geun Hyung
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201500193
Subject(s) - extracellular matrix , materials science , tissue engineering , self healing hydrogels , adipose tissue , stem cell , 3d bioprinting , biomedical engineering , cell , regeneration (biology) , nanotechnology , chemistry , microbiology and biotechnology , biochemistry , biology , engineering , polymer chemistry
Cell‐printing methods have been used widely in tissue regeneration because they enable fabricating biomimetic 3D structures laden with various cells. To achieve a cell‐matrix block, various natural hydrogels that are nontoxic, biocompatible, and printable have been combined to obtain “bioinks.” Unfortunately, most bioinks, including those with alginates, show low cell‐activating properties. Here, a strategy for obtaining highly bioactive ink, which consisted of collagen/extracellular matrix (ECM) and alginate, for printing 3D porous cell blocks is developed. An in vitro assessment of the 3D porous structures laden with preosteoblasts and human adipose stem cells (hASCs) demonstrates that the cells in the bioinks are viable. Osteogenic activities with the designed bioinks show much higher levels than with the “conventional” alginate‐based bioink. Furthermore, the hepatogenic differentiation ability of hASCs with the bioink is evaluated using the liver‐specific genes, albumin, and TDO2, under hepatogenic differentiation conditions. The genes are activated within the 3D cell block fabricated using the new bioink. These results demonstrate that the 3D cell‐laden structure fabricated using collagen/ECM‐based bioinks can provide a novel platform for various tissue engineering applications.