Premium
Electroconductive Nanopatterned Substrates for Enhanced Myogenic Differentiation and Maturation
Author(s) -
Yang Hee Seok,
Lee Bora,
Tsui Jonathan H.,
Macadangdang Jesse,
Jang SeokYoung,
Im Sung Gap,
Kim DeokHo
Publication year - 2016
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201500003
Subject(s) - nanotopography , myogenesis , myod , c2c12 , myogenin , materials science , extracellular matrix , myocyte , nanotechnology , microbiology and biotechnology , skeletal muscle , chemistry , biophysics , anatomy , biology
Electrically conductive materials provide a suitable platform for the in vitro study of excitable cells, such as skeletal muscle cells, due to their inherent conductivity and electroactivity. Here it is demonstrated that bioinspired electroconductive nanopatterned substrates enhance myogenic differentiation and maturation. The topographical cues from the highly aligned collagen bundles that form the extracellular matrix of skeletal muscle tissue are mimicked using nanopatterns created with capillary force lithography. Electron beam deposition is then utilized to conformally coat nanopatterned substrates with a thin layer of either gold or titanium to create electroconductive substrates with well‐defined, large‐area nanotopographical features. C2C12 cells, a myoblast cell line, are cultured for 7 d on substrates and the effects of topography and electrical conductivity on cellular morphology and myogenic differentiation are assessed. It is found that biomimetic nanotopography enhances the formation of aligned myotubes and the addition of an electroconductive coating promotes myogenic differentiation and maturation, as indicated by the upregulation of myogenic regulatory factors Myf5 , MyoD , and myogenin ( MyoG ). These results suggest the suitability of electroconductive nanopatterned substrates as a biomimetic platform for the in vitro engineering of skeletal muscle tissue.