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Physical Stimuli‐Induced Chondrogenic Differentiation of Mesenchymal Stem Cells Using Magnetic Nanoparticles
Author(s) -
Son Boram,
Kim Hwan D.,
Kim Minsoo,
Kim Jeong Ah,
Lee Jinkyu,
Shin Heungsoo,
Hwang Nathaniel S.,
Park Tai Hyun
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400835
Subject(s) - chondrogenesis , mesenchymal stem cell , microbiology and biotechnology , cartilage , materials science , cellular differentiation , tissue engineering , glycosaminoglycan , magnetic nanoparticles , stem cell , nanotechnology , biomedical engineering , biophysics , chemistry , anatomy , biology , nanoparticle , medicine , biochemistry , gene
Chondrogenic commitments of mesenchymal stem cells (MSCs) require 3D cellular organization. Furthermore, recent progresses in bioreactor technology have contributed to the development of various biophysical stimulation platforms for efficient cartilage tissue formation. Here, an approach is reported to drive 3D cellular organization and enhance chondrogenic commitment of bone‐marrow‐derived human mesenchymal stem cells (BM‐hMSCs) via magnetic nanoparticle (MNP)‐mediated physical stimuli. MNPs isolated from Magnetospirillum sp. AMB‐1 are endocytosed by the BM‐hMSCs in a highly efficient manner. MNPs‐incorporated BM‐hMSCs are pelleted and then subjected to static magnetic field and/or magnet‐derived shear stress. Magnetic‐based stimuli enhance level of sulfated glycosaminoglycan (sGAG) and collagen synthesis, and facilitate the chondrogenic differentiation of BM‐hMSCs. In addition, both static magnetic field and magnet‐derived shear stress applied for the chondrogenic differentiation of BM‐hMSCs do not show increament of hypertrophic differentiation. This MNP‐mediated physical stimulation platform demonstrates a promising strategy for efficient cartilage tissue engineering.