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Intracellular Delivery of Bioactive Cargos to Hard‐to‐Transfect Cells Using Carbon Nanosyringe Arrays under an Applied Centrifugal g ‐Force
Author(s) -
Choi Minsuk,
Lee Sang Ho,
Kim Won Bae,
Gujrati Vipul,
Kim Daejin,
Lee Jinju,
Kim JaeIl,
Kim Hyungjun,
Saw Phei Er,
Jon Sangyong
Publication year - 2016
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400834
Subject(s) - transfection , intracellular , cytosol , gene delivery , nanotechnology , microbiology and biotechnology , biophysics , materials science , chemistry , biology , cell culture , biochemistry , genetics , enzyme
There is considerable interest in developing a common, universal platform for delivering biomacromolecules such as proteins and RNAs into diverse cells with high efficiency. Here, it is shown that carbon nanosyringe arrays (CNSAs) under an applied centrifugal g ‐force ( cf ‐CNSAs) can deliver diverse bioactive cargos directly into the cytosol of hard‐to‐transfect cells with relatively high efficiency and reproducibility. The cf ‐CNSA platform, an optimized version of a previous CNSA‐mediated intracellular delivery platform that adds a g ‐force feature, exhibits more rapid and superior delivery of cargos to various hard‐to‐transfect cells than is the case in the absence of g ‐force. Active species, including small interfering RNAs, plasmids, and proteins are successfully transported across plasma membrane barriers into various cells. By overcoming the limitations of currently available transfection methods, the cf ‐CNSA platform paves the way to universal delivery of a variety of cargos, facilitating the analysis of cellular responses in diverse cell types.