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Nanoparticles Complexed with Gene Vectors to Promote Proliferation of Human Vascular Endothelial Cells
Author(s) -
Li Qian,
Shi Changcan,
Zhang Wencheng,
Behl Marc,
Lendlein Andreas,
Feng Yakai
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400817
Subject(s) - lipofectamine , transfection , gene delivery , genetic enhancement , in vitro , plasmid , recombinant dna , green fluorescent protein , gene transfer , amphiphile , chemistry , gene , microbiology and biotechnology , materials science , biophysics , copolymer , polymer , biochemistry , biology , vector (molecular biology) , organic chemistry
Amphiphilic block copolymers containing biodegradable hydrophobic segments of depsipeptide based copolymers have been synthesized and explored as gene carriers for enhancing proliferation of endothelial cells in vitro. These polymers form nanoparticles (NPs) with positive charges on their surface, which could condense recombinant plasmids of enhanced green fluorescent protein plasmid and ZNF580 gene (pEGFP‐ZNF580) and protect them against DNase I. ZNF580 gene is efficiently transported into EA.hy926 cells to promote their proliferation, whereby the transfection efficiency of NPs/pEGFP‐ZNF580 is approximately similar to that of Lipofectamine 2000. These results indicate that the NPs might have potential as a carrier for pEGFP‐ZNF580, which could support endothelialization of cardiovascular implants.