Premium
Tropoelastin Incorporation into a Dermal Regeneration Template Promotes Wound Angiogenesis
Author(s) -
Wang Yiwei,
Mithieux Suzanne M.,
Kong Yvonne,
Wang XueQing,
Chong Cassandra,
Fathi Ali,
Dehghani Fariba,
Panas Eleni,
Kemnitzer John,
Daniels Robert,
Kimble Roy M.,
Maitz Peter K.,
Li Zhe,
Weiss Anthony S.
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400571
Subject(s) - tropoelastin , angiogenesis , wound healing , dermis , regeneration (biology) , cd146 , neovascularization , pathology , elastin , microbiology and biotechnology , medicine , surgery , biology , cancer research , stem cell , cd34
Severe burn injury results in substantial skin loss and cannot be treated by autografts. The Integra Dermal Regeneration Template (IDRT) is the leading synthetic skin substitute because it allows for wound bed regeneration and wound healing. However, all substitutes suffer from slow blood vessel ingrowth and would benefit considerably from enhanced vascularization to nurture tissue repair. It is shown here that by incorporating the human elastic protein tropoelastin into a dermal regeneration template (TDRT) we can promote angiogenesis in wound healing. In small and large animal models comprising mice and pigs, the hybrid TDRT biomaterial and IDRT show similar contraction to autografts and decrease wound contraction compared to open wounds. In mice, TDRT accelerates early stage angiogenesis by 2 weeks, as evidenced by increased angiogenesis fluorescent radiant efficiency in live animal imaging and the expression of endothelial cell adhesion marker CD146. In the pig, a full thickness wound repair model confirms increased numbers of blood vessels in the regenerating areas of the dermis closest to the hypodermis and immediately below the epidermis at 2 weeks post‐surgery. It is concluded that including tropoelastin in a dermal regeneration template has the potential to promote wound repair through enhanced vascularization.