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Hydrogel Doped with Nanoparticles for Local Sustained Release of siRNA in Breast Cancer
Author(s) -
Segovia Nathaly,
Pont Maria,
Oliva Nuria,
Ramos Victor,
Borrós Salvador,
Artzi Natalie
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400235
Subject(s) - transfection , in vivo , oligopeptide , dendrimer , self healing hydrogels , drug delivery , nanotechnology , materials science , in vitro , chemistry , biophysics , peptide , biochemistry , biology , microbiology and biotechnology , gene , polymer chemistry
Of all the much hyped and pricy cancer drugs, the benefits from the promising siRNA small molecule drugs are limited. Lack of efficient delivery vehicles that would release the drug locally, protect it from degradation, and ensure high transfection efficiency, precludes it from fulfilling its full potential. This work presents a novel platform for local and sustained delivery of siRNA with high transfection efficiencies both in vitro and in vivo in a breast cancer mice model. siRNA protection and high transfection efficiency are enabled by their encapsulation in oligopeptide‐terminated poly(β‐aminoester) (pBAE) nanoparticles. Sustained delivery of the siRNA is achieved by the enhanced stability of the nanoparticles when embedded in a hydrogel scaffold based on polyamidoamine (PAMAM) dendrimer cross‐linked with dextran aldehyde. The combination of oligopeptide‐terminated pBAE polymers and biodegradable hydrogels shows improved transfection efficiency in vivo even when compared with the most potent commercially available transfection reagents. These results highlight the advantage of using composite materials for successful delivery of these highly promising small molecules to combat cancer.