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Activated‐Ester‐Type Photocleavable Crosslinker for Preparation of Photodegradable Hydrogels Using a Two‐Component Mixing Reaction
Author(s) -
Yanagawa Fumiki,
Sugiura Shinji,
Takagi Toshiyuki,
Sumaru Kimio,
CamciUnal Gulden,
Patel Alpesh,
Khademhosseini Ali,
Kanamori Toshiyuki
Publication year - 2015
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400180
Subject(s) - self healing hydrogels , ethylene glycol , gelatin , materials science , biocompatible material , chemical engineering , tissue engineering , mixing (physics) , polymer , polymer chemistry , chemistry , organic chemistry , biomedical engineering , composite material , medicine , physics , quantum mechanics , engineering
Photodegradable hydrogels have emerged as powerful platforms for studying and directing cellular behavior in a spatiotemporally controlled manner. Photodegradable hydrogels have previously been formed by free radical polymerizations, Michael‐type addition reactions, and orthogonal click reactions. Here, an ester‐activated photocleavable crosslinker is presented for preparing photodegradable hydrogels by means of a one‐step mixing reaction between the crosslinker and a biocompatible polymer containing amino moieties (amino‐terminated tetra‐arm poly(ethylene glycol) or gelatin). It is demonstrated that photodegradable hydrogels micropatterned by photolithography can be used to culture cells with high viability and proliferation rates. The resulting micropatterned cell‐laden structures can potentially be used to create 3D biomaterials for various tissue‐engineering applications.