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Enhanced Intracellular siRNA Delivery using Bioreducible Lipid‐Like Nanoparticles
Author(s) -
Wang Ming,
Alberti Kyle,
Varone Antonio,
Pouli Dimitria,
Georgakoudi Irene,
Xu Qiaobing
Publication year - 2014
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400039
Subject(s) - intracellular , gene knockdown , small interfering rna , nanoparticle , rna interference , biophysics , nanotechnology , degradation (telecommunications) , microbiology and biotechnology , chemistry , materials science , transfection , gene , biochemistry , biology , rna , computer science , telecommunications
A new library of lipid‐like nanoparticles (lipidoids) comprising disulfide bond is developed for siRNA delivery. Bioreducible lipidoids deliver siRNA with greater efficiency than nonbioreducible lipidoids with similar chemical structures. A siRNA release investigation, as well as an intracellular siRNA trafficking study, reveals that the degradation of bioreducible lipidoid in a strongly reductive intracellular environment boosts siRNA release and enhances siRNA gene knockdown efficiency.