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Hybrid Nanotopographical Surfaces Obtained by Biomimetic Mineralization of Statherin‐Inspired Elastin‐Like Recombinamers
Author(s) -
Li Yuping,
Chen Xi,
Ribeiro Artur J.,
Jensen Eric D.,
Holmberg Kyle V.,
RodriguezCabello J. Carlos,
Aparicio Conrado
Publication year - 2014
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201400015
Subject(s) - nanotopography , biomineralization , materials science , nanotechnology , mineralization (soil science) , enamel paint , biocompatibility , amorphous calcium phosphate , surface modification , tissue engineering , chemistry , chemical engineering , biomedical engineering , calcium , composite material , metallurgy , medicine , organic chemistry , nitrogen , engineering
Modification of surfaces mimicking unique chemical and physical features of mineralized tissues is of major interest for obtaining biomaterials for replacing and regenerating biological tissues. Here, human salivary statherin‐inspired genetically engineered recombinamers (ELRs, HSS) on biomedical surfaces regulates mineralization to form an amorphous‐calcium‐phosphate (ACP) layer that reproduces the original substrate nanotopography. The HSS‐ELRs carry a statherin‐derived peptide with high affinity to tooth enamel. They are tethered to nanorough surfaces and mineralized using an enzyme‐directed process. A homogeneous layer of ACP‐minerals forms on HSS‐coated surfaces retaining the original nanotopography of the substrate. In contrast, biomineralization of control surfaces results in uncontrolled growth of minerals. This suggest the statherin‐inspired ELRs have ability to induce and control growth of the minerals on the biofunctional surfaces. Likely, the HSS‐ELR coating have similar bioactivity to that of statherin in human saliva. The hybrid nanorough surfaces improve adhesion and differentiation of preosteoblasts and show potential for dental and orthopedic implants integration. This method enables the combination and tailoring of nanotopographical and biochemical cues to design functionalized surfaces to investigate and potentially direct the stem cell fate.