Premium
Influence of Surface‐Imprinted Nanoparticles on Trypsin Activity
Author(s) -
Guerreiro António,
Poma Alessandro,
Karim Kal,
Moczko Ewa,
Takarada Jessica,
de VargasSansalvador Isabel Perez,
Turner Nicholas,
Piletska Elena,
de Magalhães Cristiana Schmidt,
Glazova Natalia,
Serkova Anastasia,
Omelianova Aleksandra,
Piletsky Sergey
Publication year - 2014
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201300634
Subject(s) - trypsin , imprinting (psychology) , nanoparticle , enzyme , molecular imprinting , chemistry , enzyme assay , biophysics , chromatography , biochemistry , materials science , selectivity , nanotechnology , biology , catalysis , gene
Here, the modulation of enzyme activity is presented by protein‐imprinted nanoparticles produced using a solid‐phase approach. Using trypsin as target, binding of the nanoparticles to the enzyme results in its inhibition or in stabilization, depending on the orientation of the immobilized enzyme used during imprinting.