Premium
Photostimulation of Whole‐Cell Conductance in Primary Rat Neocortical Astrocytes Mediated by Organic Semiconducting Thin Films
Author(s) -
Benfenati Valentina,
Martino Nicola,
Antognazza Maria Rosa,
Pistone Assunta,
Toffanin Stefano,
Ferroni Stefano,
Lanzani Guglielmo,
Muccini Michele
Publication year - 2014
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201300179
Subject(s) - photostimulation , astrocyte , ion channel , biophysics , materials science , depolarization , membrane potential , conductance , neuroscience , chemistry , biology , central nervous system , biochemistry , receptor , mathematics , combinatorics
Astroglial ion channels are fundamental molecular targets in the study of brain physiology and pathophysiology. Novel tools and devices intended for stimulation and control of astrocytes ion channel activity are therefore highly desirable. The study of the interactions between astrocytes and biomaterials is also essential to control and minimize reactive astrogliosis, in view of the development of implantable functional devices. Here, the growth of rat primary neocortical astrocytes on the top of a light sensitive, organic polymer film is reported; by means of patch‐clamp analyses, the effect of the visible light stimulation on membrane conductance is then determined. Photoexcitation of the active material causes a significant depolarization of the astroglial resting membrane potential: the effect is associated to an increase in whole‐cell conductance at negative potentials. The magnitude of the evoked inward current density is proportional to the illumination intensity. Biophysical and pharmacological characterization suggests that the ion channel mediating the photo‐transduction mechanism is a chloride channel, the ClC‐2 channel. These results open interesting perspectives for the selective manipulation of astrocyte bioelectrical activity by non‐invasive, label‐free, organic‐based, photostimulation approaches.