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Immobilization of Actively Thromboresistant Assemblies on Sterile Blood‐Contacting Surfaces
Author(s) -
Qu Zheng,
Krishnamurthy Venkat,
Haller Carolyn A.,
Dorr Brent M.,
Marzec Ulla M.,
Hurst Sawan,
Hinds Monica T.,
Hanson Stephen R.,
Liu David R.,
Chaikof Elliot L.
Publication year - 2014
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201300110
Subject(s) - thrombomodulin , point of care , heparin , computer science , mutant , nanotechnology , biomedical engineering , chemistry , materials science , medicine , surgery , biochemistry , gene , platelet , pathology , immunology , thrombin
Rapid one‐step modification of thrombomodulin with alkylamine derivatives such as azide, biotin, and PEG is achieved using an evolved sortase (eSrtA) mutant. The feasibility of a point‐of‐care scheme is demonstrated herein to site‐specifically immobilize azido‐thrombomodulin on sterilized commercial ePTFE vascular grafts, which exhibit superior thromboresistance compared with commercial heparin‐coated grafts in a primate model of acute graft thrombosis.