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A Reusable Sensor for the Label‐Free Detection of Specific Oligonucleotides by Surface Plasmon Fluorescence Spectroscopy
Author(s) -
Nöll Gilbert,
Su Qiang,
Heidel Björn,
Yu Yaming
Publication year - 2014
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201300056
Subject(s) - oligonucleotide , fluorescence , fluorescence spectroscopy , quenching (fluorescence) , plasmon , computer science , nanotechnology , spectroscopy , materials science , surface plasmon , chemistry , optoelectronics , physics , optics , dna , biochemistry , quantum mechanics
The development of a reusable molecular beacon (MB)‐based sensor for the label‐free detection of specific oligonucleotides using surface plasmon fluorescence spectroscopy (SPFS) as the readout method is described. The MBs are chemisorbed at planar gold surfaces serving as fluorescence quenching units. Target oligonucleotides of 24 bases can be detected within a few minutes at high single‐mismatch discrimination rates.