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Delivery of Proteases in Aqueous Two‐Phase Systems Enables Direct Purification of Stem Cell Colonies from Feeder Cell Co‐Cultures for Differentiation into Functional Cardiomyocytes
Author(s) -
Frampton John P.,
Shi Huilin,
Kao Albert,
Parent Jack M.,
Takayama Shuichi
Publication year - 2013
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201300049
Subject(s) - induced pluripotent stem cell , embryonic stem cell , proteases , polyethylene glycol , peg ratio , stem cell , dextran , microbiology and biotechnology , petri dish , cell , biology , chemistry , computer science , nanotechnology , materials science , biochemistry , enzyme , genetics , gene , business , finance
Patterning of bioactive enzymes with subcellular resolution is achieved by dispensing droplets of dextran (DEX) onto polyethylene glycol (PEG)‐covered cells though a glass capillary needle connected to a pneumatic pump. This technique is applied to purify colonies of induced pluripotent stem cells (iPSCs) from mouse embryonic fibroblast (MEF) feeder cultures and inefficiently induced iPSC colonies by selectively dissociating the iPSCs with proteases.