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Two‐Photon Ratiometric Fluorescent Mapping of Intracellular Transport Pathways of pH‐Responsive Block Copolymer Micellar Nanocarriers
Author(s) -
Liu Tao,
Hu Jinming,
Jin Zhenyu,
Jin Fan,
Liu Shiyong
Publication year - 2013
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201200436
Subject(s) - copolymer , nanocarriers , fluorescence , micelle , chemistry , intracellular ph , intracellular , biophysics , nanotechnology , combinatorial chemistry , materials science , nanoparticle , polymer , organic chemistry , biochemistry , aqueous solution , optics , physics , biology
Ultrasensitive ratiometric fluorescent pH probes based on dual dye‐labeled pH‐responsive diblock copolymer micellar scaffold are constructed. The pH‐sensitive emitting nature of BTPE dyes and emission turn‐on of CMA moieties triggered by pH‐actuated micelle‐to‐unimer of diblock scaffold synergistically contribute to the observed ≈250‐fold changes of BTPE/CMA emission intensity ratios in the whole pH range. Two‐photon ratiometric fluorescent pH mapping of intracellular gradients subjected by pH‐responsive micellar nanoparticles in their endocytic pathway has been thus achieved.