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Nanowell‐Trapped Charged Ligand‐Bearing Nanoparticle Surfaces: A Novel Method of Enhancing Flow‐Resistant Cell Adhesion
Author(s) -
Tran Phat L.,
Gamboa Jessica R.,
McCracken Katherine E.,
Riley Mark R.,
Slepian Marvin J.,
Yoon JeongYeol
Publication year - 2013
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201200250
Subject(s) - adhesion , cell adhesion , nanotechnology , biomaterial , materials science , surface engineering , adhesive , nanoparticle , surface modification , substrate (aquarium) , tissue engineering , biophysics , chemistry , biomedical engineering , composite material , medicine , oceanography , biology , layer (electronics) , geology
Assuring cell adhesion to an underlying biomaterial surface is vital in implant device design and tissue engineering, particularly under circumstances where cells are subjected to potential detachment from overriding fluid flow. Cell–substrate adhesion is a highly regulated process involving the interplay of mechanical properties, surface topographic features, electrostatic charge, and biochemical mechanisms. At the nanoscale level, the physical properties of the underlying substrate are of particular importance in cell adhesion. Conventionally, natural, pro‐adhesive, and often thrombogenic, protein biomaterials are frequently utilized to facilitate adhesion. In the present study, nanofabrication techniques are utilized to enhance the biological functionality of a synthetic polymer surface, polymethymethacrylate, with respect to cell adhesion. Specifically we examine the effect on cell adhesion of combining: 1. optimized surface texturing, 2. electrostatic charge and 3. cell adhesive ligands, uniquely assembled on the substrata surface, as an ensemble of nanoparticles trapped in nanowells. Our results reveal that the ensemble strategy leads to enhanced, more than simply additive, endothelial cell adhesion under both static and flow conditions. This strategy may be of particular utility for enhancing flow‐resistant endothelialization of blood‐contacting surfaces of cardiovascular devices subjected to flow‐mediated shear.