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Biomimetic Calcium Carbonate Concentric Microgrooves with Tunable Widths for Promoting MC3T3‐E1 Cell Functions
Author(s) -
Wu Xiaohui,
Wang Shanfeng
Publication year - 2013
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201200205
Subject(s) - concentric , calcium carbonate , materials science , calcium , biophysics , biomineralization , carbonate , chemical engineering , nanotechnology , composite material , biology , geometry , mathematics , engineering , metallurgy
Abstract Biomimetic, self‐assembled calcium carbonate (CaCO 3 ) concentric microgrooves with groove widths of 5.0 and 10 μm were fabricated through simply controlling incubation temperature. Mouse pre‐osteoblastic MC3T3‐E1 cells were cultured on flat and microgrooved substrates of CaCO 3 and their adhesion, spreading, proliferation, alkaline phosphatase activity, and calcium content were remarkably enhanced by the microgrooves, in particular, the narrower ones. Furthermore, focal adhesions and actin filaments of MC3T3‐E1 cells could be aligned on both 5.0‐μm and 10‐μm‐wide CaCO 3 grooves. Compared with the original round nuclei on the flat substrates and expanded round nuclei on the narrower microgrooves, the MC3T3‐E1 cell nuclei on 10‐μm‐wide CaCO 3 grooves demonstrated preferred entrapment in the grooves and significant alignment with a smaller area after two‐day culture.