Premium
Fate of Intravenously Administered Gold Nanoparticles in Hair Follicles: Follicular Delivery, Pharmacokinetic Interpretation, and Excretion
Author(s) -
Kempson Ivan M.,
Chien ChiaChi,
Chung ChaoYu,
Hwu Yeukuang,
Paterson David,
de Jonge Martin D.,
Howard Daryl L.
Publication year - 2012
Publication title -
advanced healthcare materials
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.288
H-Index - 90
eISSN - 2192-2659
pISSN - 2192-2640
DOI - 10.1002/adhm.201200101
Subject(s) - pharmacokinetics , colloidal gold , in vivo , nanoparticle , excretory system , chemistry , biophysics , pharmacology , materials science , nanotechnology , medicine , biology , microbiology and biotechnology
Abstract Gold nanoparticles (GNPs) are intravenously administered to mice. Deposition at the pilosebacious unit and whiskers is visualized with X‐ray fluorescence after 30 minutes and 14 days. After 30 minutes the dermal papilla, bulge region, and root sheath all contain NPs. GNPs are driven externally out from follicles, counteractive to transfollicular delivery. After 14 days, gold bands in hairs reflect pharmacokinetic profiles indicating blood concentration kinetics. Elimination rate constants infer half‐lives from 3 hairs from an individual mouse within reasonable agreement (6.08, 7.15, and 8.66 hours). 3D reconstruction of NP distributions with confocal microscopy identifies aggregates within the medullary canal. Intermittent NP deposition continues randomly over the two week period demonstrating prolonged NP mobility in vivo. NPs are still retained at the hair bulb after 14 days. The observations further account for the excretory mechanisms of NPs and their behavior in the pilosebacous unit, and demonstrate monitoring pharmacokinetic behavior in individual animals.